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Tm calculator for PCR
The calculated Tm for a given primer can vary significantly between different calculation methods. The Tm calculator below utilizes the method Finnzymes has used for optimizing PCR protocols. We recommend this calculator to be used when setting up amplifications with Finnzymes' DyNAzyme, Phire and Phusion DNA Polymerases and DyNAmo qPCR Kits. See the tables below for specific directions for each product.
NOTE: If the PCR primer contains desired mismatches, e.g. for creating a mutation or a restriction site, make sure to calculate the Tm only for the correctly matched sequence.
