Welcome to the new Tm calculator! It has been improved to help you even more with your research. See our other new webtools as well!

Tm calculator

This application calculates the Tm for a primer, and gives instructions on how to dilute the primer to a desired concentration.

The application requires Java Virtual Machine.
Download Java

Important notes

If the PCR primer contains desired mismatches, e.g. for creating a mutation or a restriction site, make sure to calculate the Tm only for the correctly matched sequence.
To determinine annealing temperature for PCR, read these instructions.


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Speed. Fidelity. Yield. Specificity.

*	Breslauer et al., Proc. Nat. Acad. Sci. 83, 3746-50 (1986)
**	SantaLucia J., Proc. Nat. Acad. Sci. 95: 1460-1465 (1998)