M-MuLV Reverse Transcriptase, RNase H^-

High sensitivity enzyme for cDNA synthesis

Description

Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase RNase H^- is an RNA-directed DNA polymerase used for synthesizing a complementary DNA strand. Copying is initiated from a primer using either single-stranded RNA or single-stranded DNA template.

M-MuLV reverse transcriptase RNase H^- is purified from a strain of E.coli carrying the Reverse Transcriptase gene from M-MuLV, modified by site-directed mutagenesis to eliminate the RNase H activity. M-MuLV RNase H^- RT has improved efficiency for full length cDNA synthesis.

Applications

• cDNA synthesis from single-stranded RNA or DNA.
• Multiple transcripts can be analyzed from a single RT-reaction
• Stand alone enzymes are useful when the same cDNA source is used for analysing several different genes.

Advantages

• High sensitivity
• Improved efficiency for full length cDNA synthesis
• Purified from a recombinant source

Components

M-MuLV RT RNase H^- is supplied with 10x M-MuLV Buffer.

Reaction buffer concentrate
1x M-MuLV Reverse Transcriptase Buffer: 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl₂, 10 mM dithiothreitol.

Storage buffer
50 mM Tris-HCl (pH 7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.1 % Nonidet P40, and 50 % glycerol (v/v).

Storage and stability

Recommended storage temperature -20 °C. Stable for one year from the assay date.

Ordering information

M-MuLV Reverse Transcriptase, RNase H-	Size	Concentration
F-572S	10 000 U	200 U/µl
F-572L	50 000 U	200 U/µl