Phi6 RNA Replicase

RNA replicase for a wide range of templates.

Description

The Phi6 RNA Replicase is a modified version of the protein P2 from bacteriophage Phi6. This novel RNA-dependent RNA polymerase catalyzes the synthesis of a full-length complementary RNA strand initiating from the 3’ terminus of a single-stranded RNA. The polymerase does not require any oligonucleotide primer for the initiation. Due to a unique modification, the Phi6 RNA Replicase displays relatively low template specificity and it is therefore capable of replicating a great variety of RNA templates, as well as denatured DNA that contains a recognition sequence for the Phi6 RNA Replicase at its 3’ terminus.

Applications

• Replication of ssRNA to dsRNA form
• Synthesis and amplification of dsRNA using dsDNA as template
  Detailed protocol
• For producing larger amounts of dsRNA, we recommend the Replicator RNAi Kit

Contents

Phi6 RNA Replicase is supplied with 10x Phi6 RNA Replicase Buffer and 50 mM MnCl₂ solution. 1x buffer contains: 50 mM Tris-HCl (pH 8.75 at 21°C), 50 mM NH₄Ac.

Storage buffer

50 mM Tris-HCl (pH 8.0 at 25°C), 0.1 mM EDTA, 100 mM NaCl, 0.1 % Triton® X-100 and 50 % glycerol.

Storage

Recommended storage temperature -20 °C.

Ordering information

Phi6 RNA Replicase
F-611S	60 U (1 U / µl)
F-611L	300 U (1 U / µl)