Phi6 dsRNA

Mixture of dsRNA fragments for use as RNA control.

Description

This product is an equimolar mixture of three linear double-stranded RNA segments that constitute the bacteriophage Phi6 genome. The dsRNA fragments are 2948 bp (S segment), 4063 bp (M) and 6374 bp (L) in length. The dsRNA is isolated from the wild-type bacteriophage Phi6 propagated in P. syringae pv. phaseolicola HB10Y.

 
 

M

1

   

M

λ HindIII Digest

1

The Phi6 RNA fragments
2948 bp, 4063 bp and 6374 bp

   
 

Applications

dsRNA size standard for agarose gel electrophoresis

 

Control RNA for dsRNA-specific enzymatic reactions, e.g. Dicer, RNaseIII or RNA helicases

 
 

Contents

Phi6 dsRNA in a total concentration of 500 ng/µl in 10 mM Tris-HCl (pH 8.0), 1 mM EDTA. The relative concentrations of the S, M and L segments are approximately 110, 150 and 240 ng/µl, respectively.

Storage and stability

Recommended storage temperature -20°C. Stable for one year from the packaging date.

Ordering information

Phi6 dsRNA

F-630

20 µg (500 ng/µl)



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