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DyNAmo™ SYBR® Green qPCR Kit and
DyNAmo™ SYBR® Green qPCR Kit with ROX™ passive reference dye
Description
DyNAmo™ SYBR® Green qPCR Kit is a ready-to-use 2x master mix for sensitive, quantitative real-time PCR. The master mix contains a modified Thermus brockianus DNA polymerase and all the other reagents needed for qPCR. Only template DNA and PCR primers need to be added by the user. The modified DNA polymerase in this kit incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex. If a hot start polymerase is preferred, DyNAmo HS SYBR Green qPCR Kit is the recommended choice for block-based instruments and DyNAmo™ Capillary SYBR® Green qPCR Kit for capillary-based instruments.
In DyNAmo™ SYBR® Green qPCR Kit with ROX™ passive reference dye the ROX™ passive reference dye is supplied with the kit in a separate tube. This kit is recommended for instruments that require ROX normalization.
Advantages
- Sensitivity: Optimized 2x master mix allows reproducible detection of low-copy number templates.
- Speed: More efficient amplification coupled with increased polymerase processivity results in earlier C(t)s and shorter overall qPCR reaction times. (See figures 1 and 2) (Threshold cycle, C(t), is the cycle at which the fluorescence reaches the threshold level. The threshold level is set manually or calculated automatically.)
- Convenience: 2x master mix, one tube only. In F-400RS/L ROX passive reference dye is provided in a separate vial.
- Broad dynamic range: DyNAmo SYBR Green qPCR Kit detects as few as one copy of the target gene and achieves a dynamic range of up to eight orders of magnitude.
Sensitivity, reproducibility and speed
Amplification curves below show clearly that lower copy number detection and earlier C(t)-values are obtained with the DyNAmo SYBR Green qPCR Kit. Signal level is also considerably higher and reaction times are substantially reduced when using the DyNAmo SYBR Green qPCR Kit.
Figures 1a,2a. Amplification plot showing the linearity of the 10-fold dilutions of template as cycle numbers vs. fluorescence. Comparison of Finnzymes DyNAmo™ SYBR® Green qPCR Kit (1) and a qPCR kit from a leading supplier (2). A 221 bp PCR product was amplified from a plasmid containing a fragment of a human gene. Reactions were set up on the DNA Engine Opticon® system (Bio-Rad laboratories) according to suppliers instructions. Reaction volumes of 20 µl were used with starting template copy number ranging from 1 to 1,000,000 copies.
Figures 1b,2b. Standard curves generated from C(t) values. The curves can be used for measuring unknown amounts of sample target (the same target as used in standards).
Components
DyNAmo™ SYBR® Green qPCR Kit |
F-400S |
F-400L |
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2x Master Mix (containing modified Thermus brockianus DNA Polymerase, SYBR Green I dye, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP) |
1 x 1 ml (for 40 reactions of 50 µl volume or 100 reactions of 20 µl volume) |
5 x 1 ml (for 200 reactions of 50 µl volume or 500 reactions of 20 µl volume) |
DyNAmo™ SYBR® Green qPCR Kit with ROX™ passive reference dye |
F-400RS |
F-400RL |
|---|---|---|
2x Master Mix (containing modified Thermus brockianus DNA Polymerase, SYBR Green I dye, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP) |
1 x 1 ml (for 40 reactions of 50 µl volume or 100 reactions of 20 µl volume) |
5 x 1 ml (for 200 reactions of 50 µl volume or 500 reactions of 20 µl volume) |
50x ROX™ passive reference dye |
1 x 50 µl |
1 x 250 µl |
Storage and stability
Both DyNAmo SYBR Green qPCR Kits are shipped in gel ice. Upon arrival, store all kit components at +4 °C or -20 °C. When stored and handled properly the DyNAmo SYBR Green qPCR Kits are stable for six months in +4 °C or one year in -20 °C from the date of packaging.
Ordering information
DyNAmo™ SYBR® Green qPCR Kit |
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F-400S |
40 reactions (50 µl each) |
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F-400L |
200 reactions (50 µl each) |
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| DyNAmo™ SYBR® Green qPCR Kit with ROX™ passive reference dye | |||
F-400RS |
40 reactions (50 µl each) |
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F-400RL |
200 reactions (50 µl each) |
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| Reagents | Instruments | Consumables |
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Instruction manual for DyNAmo™ SYBR® Green qPCR Kits | ||
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Brochure for DyNAmo™ qPCR Kits | ||
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Principles of qPCR Basics of chemistries, instruments, assays and main applications Order a free copy! |
