DyNAmo™ Probe qPCR Kit

Description

DyNAmo™ Probe qPCR Kit is a ready-to-use 2x master mix for highly specific quantitative PCR. The master mix contains all reagents needed for qPCR. Only template DNA, PCR primers and probe need to be added by the user. The 2x master mix includes a hot start Thermus brockianus DNA polymerase.

ROX™ passive reference dye is also supplied with the kit in a separate tube for instruments that require ROX normalization.

DyNAmo Probe qPCR Kit is compatible with all block-based and capillary-based real-time qPCR instruments.

Advantages

Broad dynamic range and sensitivity

Human papilloma virus genome was cloned into a plasmid. Dilution series from 108 to 10 copies was prepared to be used as a standard DNA. A 76 bp fragment was amplified with F-450L DyNAmo™ Probe qPCR master mix and detected with a HEX-BHQ labeled TaqMan® probe. Reactions were setup on ABI 7500 Real Time PCR System (Applera Corporation, Inc.). The slope of the standard curve was -3.363 and the correlation coefficient R2 was 0.9997.

Components

DyNAmo™ Probe qPCR Kit

F-450S

F-450L

2x Master Mix (containing hot start Thermus brockianus DNA polymerase, optimized PCR buffer, MgCl2, dNTP mix including dUTP)

1 x 1 ml (for 40 reactions of 50 µl volume or 100 reactions of 20 µl volume)

5 x 1 ml (for 200 reactions of 50 µl volume or 500 reactions of
20 µl volume)

50x ROX™ passive reference dye

1 x 50 µl

1 x 250 µl

Storage and stability

DyNAmo Probe qPCR Kit is shipped in gel ice. Upon arrival store all kit components at -20 ºC. The DyNAmo Probe qPCR Kit is stable for six months from the date of packaging when stored and handled properly.

After use, the leftover thawed 2x master mix can be refrozen and stored at -20 °C.

Ordering information

DyNAmo™ Probe qPCR Kit

F-450S

40 reactions (50 µl each)

F-450L

200 reactions (50 µl each)
Finnzymes Oy    -   Keilaranta 16 A, 02150 Espoo, Finland    -    Tel. +358 9 2472 3010  -  Fax +358 9 2472 3200    -    fz@finnzymes.fi