DyNAmo™ HS SYBR^® Green qPCR Kit

Description

DyNAmo™ HS SYBR® Green qPCR Kit is a ready-to-use 2x master mix for highly specific quantitative PCR. The master mix contains all reagents needed for qPCR. Only template DNA and PCR primers need to be added by the user. The 2x master mix includes a hot start version of a modified Thermus brockianus DNA polymerase. The modified DNA polymerase incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex.

ROX™ passive reference dye is also supplied with the kit in a separate tube for instruments that require ROX normalization.

DyNAmo HS SYBR Green qPCR Kit is compatible with all block-based real-time qPCR instruments. The recommended kit for the users of capillary based instruments is DyNAmo Capillary SYBR Green qPCR Kit.

Advantages

• Specificity: DyNAmo HS SYBR Green master mix minimizes the formation of unwanted primer-dimers and extension of non-specifically bound primers during reaction setup.
• Sensitivity: Optimized master mix allows reproducible detection of low-copy number templates.
• Broad dynamic range: DyNAmo HS SYBR Green qPCR Kit detects as few as one copy of the target gene and achieves a dynamic range of up to eight orders of magnitude.
• Convenience: 2x master mix, one tube only. ROX passive reference dye provided in a separate vial.

DyNAmo™ HS SYBR^® Green qPCR Kit
- specificity and sensitivity

Amplification and melting curves below (Figures 1 and 2) show that DyNAmo HS SYBR Green qPCR Kit has an excellent performance over a broad range of template copy numbers, and amplifies a specific product even from a difficult template.

Figures 1a-1b. A 335 bp PCR product was amplified from lambda DNA with DyNAmo HS SYBR Green qPCR Kit. A reaction volume of 20 µl was used with starting template copy number ranging from 5 to 50,000,000 copies. Reactions were set up on DNA Engine Opticon® System (Bio-Rad Laboratories).

 

Figures 2a-2c. A 481 bp amplicon of a beta actin gene was amplified with DyNAmo HS SYBR Green qPCR Kit. Reactions were set up on the DNA Engine Opticon system (Bio-Rad Laboratories). Reaction volumes of 20 µl were used with starting template amount ranging from 0.035 ng to 200 ng. The melting curve shows that the kit has amplified the fragment with excellent specificity.

Components

DyNAmo™ HS SYBR® Green qPCR Kit	F-410S	F-410L
2x Master Mix (containing a hot start version of a modified Thermus brockianus DNA polymerase, SYBR Green I dye, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP)	1 x 1 ml (for 40 reactions of 50 µl volume or 100 reactions of 20 µl volume)	5 x 1 ml (for 200 reactions of 50 µl volume or 500 reactions of 20 µl volume)
50x ROX passive reference dye	1 x 50 µl	1 x 250 µl

Storage and stability

The DyNAmo HS SYBR Green qPCR Kit is shipped in gel ice. Upon arrival store all kit components at -20 ºC. The kit is stable for six months from the date of packaging when stored and handled properly.

After use, the leftover thawed 2x master mix can be refrozen and stored at -20 °C.

Ordering information

DyNAmo™ HS SYBR® Green qPCR Kit
F-410S	40 reactions (50 µl each)
F-410L	200 reactions (50 µl each)