DyNAmo™ Flash SYBR^® Green qPCR Kit

Description

Finnzymes’ novel DyNAmo™ Flash SYBR® Green qPCR Kit is developed for fast real-time qPCR. It provides qPCR results faster than other SYBR Green kits without compromising the qPCR performance. A DNA-binding domain attached to the polymerase in this kit improves the physical stability of the polymerase-DNA complex, thus enhancing the processivity and robustness of the amplification. The high amplification efficiency of DyNAmo Flash SYBR Green qPCR Kit gives reliable quantitation and early C(t) values. Due to the high signal intensity, DyNAmo Flash SYBR Green qPCR provides an excellent signal-to-noise ratio.

Advantages

• qPCR results faster without compromising performance
• Reliable and robust amplification
• High specificity and sensitivity
• High signal intensity

Fast real-time qPCR

Below is a comparison of qPCR cycling times between SYBR Green qPCR kits from different suppliers. All protocols were conducted as recommended by the manufacturers and set up on the DNA Engine Opticon® 2 system (Bio-Rad Laboratories, Inc). With the new DyNAmo Flash SYBR Green qPCR Kit, the cycling time can be reduced by as much as 92 min compared to other SYBR Green kits.

Efficient amplification and high signal intensity

Amplification of a 141 bp cDNA amplicon fron human calmodulin gene with DyNAmo Flash SYBR Green qPCR Kit (A; combined annealing and extension 15 s) and a kit from another supplier (B; annealing 30 s + extension 30 s). Reaction and cycling protocols recommended by the suppliers were followed. DyNAmo Flash SYBR Green qPCR Kit delivers efficient amplification and higher signal intensity (note different fluorescence scales in A and B).

Components

DyNAmo™ Flash SYBR® Green qPCR Kit	F-415S	F-415L
2x master mix (contains hot start version of a modified Tbr DNA polymerase, SYBR Green I, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP)	1 x 1ml (sufficient for 40 reactions of 50 µl or 100 reactions of 20 µl)	5  x 1ml (sufficient for  200 reactions of 50 µl or 500 reactions of 20 µl)
50x ROX passive reference dye	1 x 50 µl	1 x 250 µl

Storage and stability

DyNAmo Flash SYBR Green qPCR Kit is shipped on gel ice. Upon arrival, store all kit components at -20 °C. When using the 2x master mix, the leftover thawed mix can be refrozen and stored at -20 °C without affecting the performance of the kit.

The kit is stable for six months from the date of packaging when stored and handled properly.

Ordering information

DyNAmo™ Flash SYBR® Green qPCR Kit
F-415S	40 reactions (50 µl each) or 100 reactions (20 µl each)
F-415L	200 reactions (50 µl each) or 500 reactions (20 µl each)