DyNAmo™ cDNA Synthesis Kit

Description

Advantages

Kit Components

Storage and stability

Ordering information

for qRT-PCR

The DyNAmo™ cDNA Synthesis Kit is intended for cDNA synthesis for two-step quantitative reverse transcription-PCR (qRT-PCR) applications, where amplicons are usually around 100 bp in length. This kit is a part of Finnzymes' DyNAmo 2-step qRT-PCR Kits and it can be used in conjunction with DyNAmo qPCR Kits or with any other qPCR kit suitable for the application.

The DyNAmo cDNA Synthesis Kit includes all necessary reagents for cDNA synthesis to be used in qPCR. Either total RNA, messenger RNA, viral RNA or in vitro transcribed RNA can be used as a template for reverse transcription. The kit includes both random primers and oligo(dT) primers. The user can choose either of these or alternatively use gene-specific primers.The reverse transcriptase in the kit is M-MuLV RNase H⁺, which provides higher sensitivity to qPCR than RNase H^- reverse transcriptases.

Broad dynamic range: up to 1 µg of RNA
Reliable results with different RNA sources: mRNA, total RNA, viral RNA and in vitro transcribed RNA
Flexible: all priming options for cDNA synthesis
Optimized 2x mix for cDNA synthesis
Convenient to use: short protocol

DyNAmo™ cDNA Synthesis Kit	F-470S	F-470L
M-MuLV RNase H⁺ reverse transcriptase (includes RNase inhibitor)	1 x 40 µl (sufficient for 20 RT reactions of 20 µl volume)	1 x 200 µl (sufficient for 100 RT reactions of 20 µl volume)
2x RT buffer (includes dNTP mix and 10 mM MgCl₂)	1 x 200 µl	1 x 1 ml
Random hexamers (300 ng/µl)	1 x 20 µl	1 x 100 µl
Oligo(dT)₁₅ primer (100 ng/µl)	1 x 20 µl	1 x 100 µl

DyNAmo cDNA Synthesis Kit is shipped in gel ice. Upon arrival, store all kit components at -20 °C. All the kit components can be refrozen and stored at -20 °C without affecting the performance of the kit.

The kit is stable for one year from the date of packaging when stored and handled properly.

Instruments

Consumables

DyNAmo™ cDNA Synthesis Kit
F-470S	Sufficient for 20 cDNA synthesis reactions (20 µl each)
F-470L	Sufficient for 100 cDNA synthesis reactions (20 µl each)

Introduction to DyNAmo™ qPCR kits DyNAmo™ qPCR Kit compatibility with different real-time instruments DyNAmo™ qPCR Kit references Technical appendix – qPCR and qRT-PCR FAQ – qPCR and qRT-PCR


			Instruction manual for DyNAmo™ cDNA Synthesis Kit

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DyNAmo™ qPCR Kits