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DyNAmo™ cDNA Synthesis Kit
for qRT-PCR
Description
The DyNAmo™ cDNA Synthesis Kit is intended for cDNA synthesis for two-step quantitative reverse transcription-PCR (qRT-PCR) applications, where amplicons are usually around 100 bp in length. This kit is a part of Finnzymes' DyNAmo 2-step qRT-PCR Kits and it can be used in conjunction with DyNAmo qPCR Kits or with any other qPCR kit suitable for the application.
The DyNAmo cDNA Synthesis Kit includes all necessary reagents for cDNA synthesis to be used in qPCR. Either total RNA, messenger RNA, viral RNA or in vitro transcribed RNA can be used as a template for reverse transcription. The kit includes both random primers and oligo(dT) primers. The user can choose either of these or alternatively use gene-specific primers.The reverse transcriptase in the kit is M-MuLV RNase H+, which provides higher sensitivity to qPCR than RNase H- reverse transcriptases.
Advantages
- Broad dynamic range: up to 1 µg of RNA
- Reliable results with different RNA sources: mRNA, total RNA, viral RNA and in vitro transcribed RNA
- Flexible: all priming options for cDNA synthesis
- Optimized 2x mix for cDNA synthesis
- Convenient to use: short protocol
Kit Components
DyNAmo™ cDNA Synthesis Kit |
F-470S |
F-470L |
|---|---|---|
M-MuLV RNase H+ reverse transcriptase (includes RNase inhibitor) |
1 x 40 µl |
1 x 200 µl |
| 2x RT buffer (includes dNTP mix and 10 mM MgCl2) | 1 x 200 µl | 1 x 1 ml |
| Random hexamers (300 ng/µl) | 1 x 20 µl | 1 x 100 µl |
| Oligo(dT)15 primer (100 ng/µl) | 1 x 20 µl | 1 x 100 µl |
Shipping and storage
DyNAmo cDNA Synthesis Kit is shipped in gel ice. Upon arrival, store all kit components at -20 °C. All the kit components can be refrozen and stored at -20 °C without affecting the performance of the kit.
Ordering information
DyNAmo™ cDNA Synthesis Kit |
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|---|---|
F-470S |
Sufficient for 20 cDNA synthesis reactions (20 µl each) |
F-470L |
Sufficient for 100 cDNA synthesis reactions (20 µl each) |
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| Reagents | Instruments | Consumables |
