High Performance PCR:
Robust amplification even with
0 s extension time
High Performance PCR: |
|
| A 400 bp amplicon of lambda DNA was amplified with Phusion Flash High-Fidelity PCR Master Mix using three different extension times. |
| Cycling protocol (2-Step) | |||
|---|---|---|---|
| Initial denaturation | 98°C | 10 s | |
| Denaturation | 98°C | 0 s | 30 cycles |
| Annealing + extension | 72°C | 10s / 5 s / 0 s | |
| Final extension | 72°C | 1 min | |