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Phusion® DNA Polymerase products
Thermo Scientific Phusion® DNA Polymerases offer PCR performance that no other enzyme can match.
Thermo Scientific Phusion® products
Phusion® Hot Start II High-Fidelity DNA Polymerase (F-549)
Phusion® High-Fidelity DNA Polymerase (F-530)
Phusion® Flash High-Fidelity PCR Master Mix (F-548)
Phusion® High-Fidelity PCR Master Mix (F-531, F-532)
Phusion® High-Fidelity PCR Kit (F-553)
Buffer packs for Phusion® DNA Polymerases
Phusion® Blood Direct PCR Kit (F-547)
Phusion® Site-Directed Mutagenesis Kit (F-541)
Phusion® RT-PCR Kit (F-546)
Phusion® Bacterial Profiling Kit
Description
In Thermo Scientific Phusion® High-Fidelity DNA Polymerases, a unique dsDNA-binding domain is fused to a Pyrococcus-like proofreading polymerase. This domain increases the affinity of Phusion DNA Polymerases for double-stranded DNA, allowing incorporation of more nucleotides per binding event and decreasing the number of binding events required for elongation. Due to the novel fusion technique, Phusion DNA Polymerases generate PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. In addition, Phusion DNA Polymerases are capable of amplifying long amplicons (e.g. the 7.5 kb human genomic and 20 kb λ DNA used in Finnzymes’ quality control assays).
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Figure 1. The schematic structure of Phusion® High-Fidelity DNA Polymerase. The dsDNA-binding domain (purple) is fused to a novel Pyrococcus-like enzyme (green) forming a unique high-performance polymerase - Phusion DNA Polymerase. |
The processivity of Phusion DNA Polymerases is approximately 10-fold greater than that of Pyrococcus furiosus DNA polymerase and twice that of Thermus aquaticus DNA polymerase. This dramatic increase in processivity results not only in shorter extension times, but more robust amplification and the ability to amplify long templates in a fraction of the time. Phusion DNA Polymerases also produce higher yields with lower enzyme amounts than traditional proofreading polymerases. Due to these unique characteristics, Phusion DNA Polymerases can be used for routine PCR as well as for demanding PCR applications.
Advantages
Accuracy - The highest fidelity of any available thermostable polymerases
Speed - Increased processivity allows shorter reaction times (extension 15-30 s/kb)
Robustness - Fewer reaction failures and minimal optimization
High yields - Increase product yields with minimal enzyme amounts
Specificity - Hot start modification reduces non-specific amplification and primer degradation
Applications
High-fidelity PCR
The error rate of Phusion DNA Polymerase is 4.4 x 10-7, which is industry-leading fidelity. Therefore, Phusion DNA Polymerase is suitable for all PCR applications requiring great accuracy.
Cloning
Phusion DNA Polymerase amplifies templates with an accuracy and speed previously unattainable with a single enzyme. This makes it a superior choice for cloning. Phusion DNA Polymerase produces blunt ends to the amplified fragment.
Long amplicons
Extension and overall cycling times can be significantly reduced when using Phusion DNA Polymerase. Due to the high processivity, Phusion DNA Polymerase can amplify long templates in a fraction of the time other polymerases need.
DHPLC and Microarray applications
Detergent-free reaction buffers are available for Phusion DNA Polymerase. PCR products used for DHPLC analysis are recommended to be free of detergents. Detergents may also cause foaming when PCR products are spotted on microarray slides.
Application note: Direct PCR from blood preserved on Whatman FTA® and 903® Cards
Application note: Amplification of whole human mitochondrial DNA
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| No need for DNA purification | ||||||
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| Speed. Fidelity. Yield. Specificity. |