Phusion^® Hot Start High-Fidelity DNA Polymerase

The most accurate DNA polymerase now available with a hot start modification. Phusion Hot Start DNA Polymerase is part of Thermo Scientific High Performance PCR.

An upgraded version of the polymerase is available. See Thermo Scientific Phusion® Hot Start II High-Fidelity DNA Polymerase.

Description

The unique Thermo Scientific Phusion® High-Fidelity DNA Polymerase with a dsDNA-binding domain is now available with a hot start modification. The new Affibody®-based inactivation method of Phusion DNA Polymerase increases the specificity of PCR amplification by preventing non-specific extension of DNA substrate at ambient temperatures prior to the first cycle of PCR. In addition to the extreme fidelity, speed and robustness, Phusion Hot Start DNA Polymerase now also delivers extreme specificity to PCR experiments. Protocols optimized for the original Phusion High-Fidelity DNA Polymerase can be directly applied to Phusion Hot Start DNA Polymerase reactions.

General information about Thermo Scientific Phusion DNA Polymerases, their advantages and applications can be found on Phusion DNA Polymerase products page.

Contents

Phusion Hot Start High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl₂ solution. Both Phusion HF Buffer and Phusion GC Buffer contain 7.5 mM MgCl₂ in the provided 5x concentration.

Storage buffer
20 mM Tris-HCl (pH 7.4 at 25°C), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, 200 µg/ml BSA and 50% glycerol.

Storage

Recommended storage temperature -20°C.

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