Phusion^® High-Fidelity PCR Master Mix

2x master mixes with extreme performance for all PCR applications. Phusion High-Fidelity PCR Master Mix is part of Finnzymes' High Performance PCR!

Description

Phusion® High-Fidelity PCR Master Mix with HF Buffer and Phusion High-Fidelity PCR Master Mix with GC Buffer are convenient 2x mixes containing Phusion DNA Polymerase, nucleotides and optimized HF or GC reaction buffer including MgCl₂. Only template and primers need to be added by the user.

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10^-7) is lower than that in GC Buffer (9.5 x 10^-7). Therefore, the Master Mix with HF Buffer should be used as a default for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, i.e. GC-rich templates or those with complex secondary structures.

General information about Phusion DNA Polymerases, their advantages and applications can be found on Phusion DNA Polymerase products page.

Contents

2x Phusion High-Fidelity PCR Master Mix with HF Buffer contains Phusion DNA Polymerase, 2x Phusion HF Buffer and 400 µM of each dNTP. The master mix supplies 1.5 mM MgCl₂ and 200 µM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product.

2x Phusion High-Fidelity PCR Master Mix with GC Buffer contains Phusion DNA Polymerase, 2x Phusion GC Buffer and 400 µM of each dNTP. The master mix supplies 1.5 mM MgCl₂ and 200 µM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product.

Storage and stability

Recommended storage temperature -20°C. Stable for one year from the packaging date. After thawing the mix can be stored at +4°C for three months.

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