Phusion^® High-Fidelity PCR Master Mix

2x master mixes with extreme performance for all PCR applications. Phusion High-Fidelity PCR Master Mix is part of Thermo Scientific High Performance PCR!

Description

Thermo Scientific Phusion® High-Fidelity PCR Master Mix with HF Buffer and Thermo Scientific Phusion High-Fidelity PCR Master Mix with GC Buffer are convenient 2x mixes containing Phusion DNA Polymerase, nucleotides and optimized HF or GC reaction buffer including MgCl₂. Only template and primers need to be added by the user.

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10^-7) is lower than that in GC Buffer (9.5 x 10^-7). Therefore, the Master Mix with HF Buffer should be used as a default for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, i.e. GC-rich templates or those with complex secondary structures.

General information about Thermo Scientific Phusion DNA Polymerases, their advantages and applications can be found on Phusion DNA Polymerase products page.

Contents

2x Phusion High-Fidelity PCR Master Mix with HF Buffer contains Phusion DNA Polymerase, 2x Phusion HF Buffer and 400 µM of each dNTP. The master mix supplies 1.5 mM MgCl₂ and 200 µM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product.

2x Phusion High-Fidelity PCR Master Mix with GC Buffer contains Phusion DNA Polymerase, 2x Phusion GC Buffer and 400 µM of each dNTP. The master mix supplies 1.5 mM MgCl₂ and 200 µM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product.

Storage

Recommended storage temperature -20°C.

Ordering information

Phusion® High-Fidelity PCR Master Mix with HF Buffer	Size
F-531S	100 reactions in 50 µl volume
F-531L	500 reactions in 50 µl volume
Phusion® High-Fidelity PCR Master Mix with GC Buffer	Size
F-532S	100 reactions in 50 µl volume
F-532L	500 reactions in 50 µl volume