Phire^® Hot Start DNA Polymerase

Speed and specificity for routine and high-throughput PCR.

An upgraded version of the polymerase is available. See Phire® Hot Start II DNA Polymerase.

Description

Finnzymes’ Phire® Hot Start DNA Polymerase is a novel PCR enzyme that outperforms every Taq-based hot start polymerase on the market. This is a result of the polymerase’s DNA-binding domain which shortens extension times, improves yields, and increases fidelity 2-fold compared to Taq polymerase. In addition, our unique hot start technology allows complete reactivation of Phire Hot Start DNA Polymerase in “zero-time” at standard cycling temperatures. This combination of features makes this polymerase an ideal solution for routine and high throughput PCR applications. Phire DNA Polymerase delivers superior performance in all thermal cyclers, both conventional and fast instruments such as the Piko® Thermal Cycler from Finnzymes.

Figure 1. Phire Hot Start DNA Polymerase is constructed by fusing a DNA polymerase (orange) and a small dsDNA-binding protein (yellow). This technology increases the processivity of the polymerase and improves its overall performance.

Advantages

• Quick hot start: Zero-time reactivation
• Fast enzyme: Amplify three times faster than with hot start Taq
• High yields: Abundant products due to high efficiency
• Longer PCR products: Amplify significantly longer DNA fragments than with any hot start Taq

Quick hot start

Phire Hot Start DNA Polymerase utilizes Affibody® inactivation technology, which reversibly inactivates the DNA polymerase at room temperature. At polymerization temperature the Affibody ligand is released from the DNA polymerase. This completely eliminates the need for a DNA polymerase reactivation step in cycling protocol, thus speeding up PCR even more.

Fast PCR with high yields

The DNA-binding domain of Phire Hot Start DNA Polymerase stabilizes the polymerase-DNA complex during extension steps in PCR. This enables the polymerase to amplify DNA three times faster than standard Taq DNA polymerase. The high efficiency also results in abundant yields.

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Comparison of PCR cycling times	Abundant yields in shorter time

Longer PCR products

Phire Hot Start DNA Polymerase is capable of amplifying longer DNA fragments than any hot start Taq DNA polymerase on the market.

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Amplification of long fragments

Contents

Phire Hot Start DNA Polymerase is supplied with 5x Phire Reaction Buffer and DMSO. The buffer contains 7.5 mM MgCl₂ in the provided 5x concentration.

Storage and stability

Recommended storage temperature -20°C. Stable for one year from the assay date

Ordering information