Abundant yields in shorter time

A 1.5 kb fragment from the human Cathepsin K gene was amplified with five different hot start DNA polymerases according to suppliers’ recommendations. Phire® Hot Start II DNA Polymerase amplified high amounts of specific PCR product in just 29 minutes. In contrast, the PCR protocols for hot start Taq DNA polymerases from four major suppliers (A-D) were substantially longer and resulted in lower product yields.