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PCR fidelity calculator
Please enter the following values to determine the percentage of PCR products containing an error when amplified with Phusion® High-Fidelity DNA Polymerase
| Estimated percentage of PCR products having an error (i.e. DNA molecules with 1 error): |
Examples
How many percent of the product molecules contain an error after PCR (30 cycles) with different polymerases
Polymerase |
1 kb template |
3 kb template |
|---|---|---|
Phusion® High-Fidelity DNA Polymerases (HF Buffer) |
1.32 % | 3.96 % |
PhusionT High-Fidelity DNA Polymerases (GC Buffer) |
2.85 % |
8.55 % |
Pyrococcus furiosus DNA polymerase |
8.4 % |
25.2 % |
Taq DNA polymerase |
68.4 % |
205.2 % |
The table above demonstrates the low error rate
of Phusion DNA Polymerase. After 30 cycles of PCR
amplifying a 3 kb template, only 3.96 % of the
product DNA molecules contain 1 (nucleotide)
error each. This means that 96.04 % of the
product molecules are entirely error-free. In
contrast, after the same PCR protocol performed
with Taq DNA polymerase, every product molecule contains in average 2 errors.
