DyNAzyme™ II Hot Start DNA Polymerase

Recombinant thermostable DNA polymerase for hot start PCR

Description

DyNAzyme II Hot Start DNA Polymerase is a modified form of the DyNAzyme™ II DNA Polymerase originating from Thermus brockianus. DyNAzyme II Hot Start DNA Polymerase is inactive at room temperature, and the activity is recovered by a 10-minute incubation step at 94 °C. The enzyme improves the specificity of PCR by preventing the extension of non-specifically bound primers during reaction setup and the first heating cycle. The use of DyNAzyme II Hot Start DNA Polymerase is also beneficial when using primers that are prone to primer-dimer formation or when using robotic PCR systems that include incubations at room temperature prior to temperature cycling. DyNAzyme II Hot Start DNA Polymerase possesses both 5’→3’ DNA polymerase and 5’→3’ exonuclease activities. The polymerase amplifies DNA targets up to 3 kb, but optimal results are obtained with amplicons up to 1 kb.

Applications

• Routine PCR
• Robotic PCR systems
• Microsatellite amplification
• PCR with primers that are prone to primer-dimer formation
• DHPLC

Advantages

• Increased specificity
• Prevents the formation of primer-dimers
• Reaction setup can be done at room temperature

Contents

DyNAzyme II Hot Start DNA Polymerase is supplied with a 10x buffer. 1x reaction buffer contains 15 mM Tris-HCl (pH 8.2 at 25 °C), 30 mM KCl, 5 mM (NH₄)₂SO₄, 2.5 mM MgCl₂ and 0.02 % bovine serum albumin.

Storage buffer

20 mM Tris-HCl (pH 9.0 at 25 °C), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers and 50 % glycerol.

Storage and stability

Recommended storage temperature -20°C. Stable for six months from the assay date.

Ordering information

DyNAzyme™ II Hot Start DNA Polymerase	Size	Concentration
F-504S	250 U	2 U/µl
F-504L	1 000 U	2 U/µl