DyNAzyme™ II Hot Start DNA Polymerase

Recombinant thermostable DNA polymerase for hot start PCR

Note that F-504S and F-504L will be discontinued on March 31, 2012. We recommend alternative products: Maxima Hot Start Taq DNA Polymerase or Phire Hot Start II DNA Polymerase. Please contact your local representative for a free sample.

Full list of products to be discontinued.

Description

DyNAzyme II Hot Start DNA Polymerase is a modified form of the DyNAzyme™ II DNA Polymerase originating from Thermus brockianus. DyNAzyme II Hot Start DNA Polymerase is inactive at room temperature, and the activity is recovered by a 10-minute incubation step at 94 °C. The enzyme improves the specificity of PCR by preventing the extension of non-specifically bound primers during reaction setup and the first heating cycle. The use of DyNAzyme II Hot Start DNA Polymerase is also beneficial when using primers that are prone to primer-dimer formation or when using robotic PCR systems that include incubations at room temperature prior to temperature cycling. DyNAzyme II Hot Start DNA Polymerase possesses both 5’→3’ DNA polymerase and 5’→3’ exonuclease activities. The polymerase amplifies DNA targets up to 3 kb, but optimal results are obtained with amplicons up to 1 kb.

Applications

Routine PCR
Robotic PCR systems
Microsatellite amplification
PCR with primers that are prone to primer-dimer formation
DHPLC

Advantages

Increased specificity
Prevents the formation of primer-dimers
Reaction setup can be done at room temperature

DyNAzyme II Hot Start DNA Polymerase is supplied with a 10x buffer. 1x reaction buffer contains 15 mM Tris-HCl (pH 8.2 at 25 °C), 30 mM KCl, 5 mM (NH₄)₂SO₄, 2.5 mM MgCl₂ and 0.02 % bovine serum albumin.