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DyNAzyme™ II DNA Polymerase Kit
Complete kit with a control protocol for standard PCR
Description
DyNAzyme™ II DNA Polymerase Kit is available in two sizes. The small kit (F-551S) contains all necessary reagents for 100 amplifications of 50 µl each and the large kit (F-551L) contains reagents for 500 amplifications. The kits also contain control template (λ DNA) and primers for 40 control reactions. A ready to use DNA Size Standard (λ DNA-Hind III and ΦX174 DNA- Hae III digest) sufficient for 40 lanes is also included in the kits.
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To check the performance of the PCR reaction, an amplification of 500 bp of λ DNA can be made with the control template and the control primers included in the DyNAzyme™ DNA Polymerase Kits. The complete control protocol is included in the kits. The picture shows the result of the control reaction: |
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DyNAzyme™ II DNA Polymerase Kit components
Component |
Concentration |
F-551S |
F-551L |
|---|---|---|---|
DyNAzyme™ II DNA Polymerase |
2 U/µl |
100 U |
500 U |
dNTP Mix |
10 mM/each |
100 µl |
500 µl |
Optimized DyNAzyme™ Buffer |
1 x 1.5 ml |
2 x 1.5 ml |
|
Mg2+-free DyNAzyme™ Buffer |
1 x 1.5 ml |
2 x 1.5 ml |
|
MgCl2-solution |
50 mM |
1 x 1.5 ml |
1 x 1.5 ml |
Control Template (λ DNA) |
0.5 ng/µl |
40 µl |
40 µl |
500 bp primers |
25 µM/each |
40 µl |
40 µl |
DNA Size Standard (λ DNA Hind III + ΦX174 DNA Hae III digests) |
50 ng/µl of each |
400 µl |
400 µl |
Storage and stability
Recommended storage temperature -20 °C. Stable for one year from the packaging date.
Ordering information
| DyNAzyme™ II DNA Polymerase Kit | Size |
|---|---|
F-551S |
100 reactions in 50 µl volume |
F-551L |
500 reactions in 50 µl volume |
