DyNAzyme™ II DNA Polymerase

Recombinant thermostable DNA polymerase for standard PCR

Description

Thermostable DyNAzyme™ II DNA Polymerase is isolated and purified from an E. coli strain carrying a plasmid with the cloned DyNAzyme DNA Polymerase gene from Thermus brockianus, which is a Finnzymes proprietary strain. DyNAzyme II DNA Polymerase has a half life of 2.5 hours at 96 °C. It lacks 3´--> 5´proofreading activity but does contain 5´--> 3´ exonuclease activity. DyNAzyme II DNA Polymerase is purified free of contaminating endo- and exonucleases.

Advantages

• Better thermal stability than that of Taq DNA polymerase
• Tolerates DMSO better than Taq DNA polymerase
• High purity, no contaminating activities
• Supplied with optimized buffer or with Mg²⁺-free buffer and MgCl₂ solution
• High batch-to-batch consistency
• Easy to use and optimize
• Detergent-free buffer also available
  

Contents

DyNAzyme II DNA Polymerase is supplied with 10x Optimized DyNAzyme™ Buffer. 1x buffer contains:
10 mM Tris-HCl (pH 8.8 at 25°C), 1.5 mM MgCl₂, 50 mM KCl, 0.1 % Triton^® X-100.

Storage buffer

20 mM Tris-HCl (pH 7.4 at 25 °C), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, 200 µg/ml BSA and 50 % glycerol.

Storage and stability

Recommended storage temperature -20 °C. Stable for one year from the assay date.

Ordering information

DyNAzyme™ II DNA Polymerase with Optimized DyNAzyme™ Buffer	Size	Concentration
F-501S	250 U	2 U/ µl
F-501L	1000 U	2 U/ µl