DyNAzyme™ I DNA Polymerase

Native thermostable DNA polymerase from Thermus brockianus

Description

Native thermostable DyNAzyme™ I DNA Polymerase is isolated and purified from Thermus brockianus, which is a Finnzymes proprietary strain. DyNAzyme I DNA Polymerase has a half-life of 3 hours at 96 °C and it also contains 5’-->3’ exconuclease activity. DyNAzyme I DNA Polymerase is purified free of contaminating endo- and exonucleases.

Advantages

• Better thermal stability than that of Taq DNA polymerase
• Tolerates DMSO better than Taq DNA polymerase
• High purity, no contaminating activities
• Supplied with optimized buffers or with Mg²⁺-free buffers
• High batch-to-batch consistency
• Easy to use and optimize
• Detergent-free buffer also available
  

Contents

DyNAzyme™ I DNA Polymerase is supplied with 10x Optimized DyNAzyme™ Buffer. 1x reaction buffer contains: 10 mM Tris-HCl, (pH 8.8 at 25 °C), 1.5 mM MgCl₂, 50 mM KCl, 0.1 % Triton® X-100.

Storage buffer

20 mM Tris-HCl (pH 7.4 at 25 °C), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, 200 µg/ml BSA and 50 % glycerol.

Storage and stability

Recommended storage temperature -20 °C. Stable for one year from the assay date.

Ordering information

DyNAzyme™ I DNA Polymerase with optimized buffer	Size	Concentration
F-500S	100 U	2 U/ µl
F-500L	500 U	2 U/ µl