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Thermo Scientific Finnzymes DNA polymerases

Thermo Scientific product offering for PCR includes a variety of DNA polymerases providing a suitable enzyme for all purposes, from routine PCR to the most demanding high-fidelity applications. The tables below indicate the suitability of different enzymes to specific applications, and some characteristics of the polymerases.

DNA polymerase selection guide

APPLICATIONS
  Phusion® Flash High-Fidelity DNA Polymerase Phusion® Hot Start II High-Fidelity DNA Polymerase Phusion® High-Fidelity DNA Polymerase Phire® Hot Start II DNA Polymerase DyNAzyme™ EXT DNA Polymerase DyNAzyme™ II Hot Start DNA Polymerase DyNAzyme™ II DNA Polymerase DyNAzyme™ I DNA Polymerase
Direct PCR +++ +++ +++ +++
Fast PCR +++ +++ +++ +++ + + + +
High-fidelity
applications
+++ +++ +++ + ++ + + +
Cloning +++1 +++1 +++1 +1 ++ + + +
Difficult
templates
+++ +++ +++ ++ +++ + + +
Long-range PCR +++ +++ +++ +++ +++ + ++ ++
Routine PCR ++ ++ ++ +++ +++ +++ +++ ++
Microarray + +++2 +++2 +++2 +++2 +++ ++2 ++2

Site-directed
mutagenesis3

+++ +++ +++ + ++ + + +

DHPLC2

+ +++2 +++2 +++2 +++2 +++ ++2 ++2

CHARACTERISTICS
  Phusion® Flash High-Fidelity DNA Polymerase Phusion® Hot Start II High-Fidelity DNA Polymerase Phusion® High-Fidelity DNA Polymerase Phire® Hot Start II DNA Polymerase DyNAzyme™ EXT DNA Polymerase DyNAzyme™ II Hot Start DNA Polymerase DyNAzyme™ II DNA Polymerase DyNAzyme™ I DNA Polymerase
5'-3' polymerase
activity
Yes Yes Yes Yes Yes Yes Yes Yes
5'-3' exonuclease
activity
No No No No Yes Yes Yes Yes
3'-5' exonuclease
activity
Yes Yes Yes Weak Weak No No No
Reverse
transcriptase
activity
No No No No Low Low Low Low
dUTP
incorporation
No No No No No Yes Yes Yes
Recommended
extension time
15 s/kb 15-30 s/kb 15-30 s/kb 10-15 s/kb 40 s/kb 60 s/kb 40 s/kb 40 s/kb
  1. Phusion and Phire DNA polymerases generate blunt end PCR products.
    Click here for cloning instructions for Thermo Scientific DNA Polymerases
  2. Detergent-free reaction buffers are available separately for microarray and DHPLC applications, where PCR products are recommended to be free of detergents.
    Click here for more information on Polymerase Buffers
  3. For site directed mutagenesis, Finnzymes offers a complete kit:
    Phusion® Site-Directed Mutagenesis Kit

Direct links to DNA polymerase products

Phusion® Flash High-Fidelity PCR Master Mix

DyNAzyme™ EXT DNA Polymerase
DyNAzyme™ EXT PCR Kit
Phusion® Hot Start II High-Fidelity DNA Polymerase DyNAzyme™ II Hot Start DNA Polymerase
Phusion® High-Fidelity DNA Polymerase
Phusion® High-Fidelity PCR Kit
Phusion® High-Fidelity PCR Master Mix
DyNAzyme™ II DNA Polymerase
DyNAzyme™ II DNA Polymerase with Mg2+-free buffer
DyNAzyme™ II DNA Polymerase Kit
DyNAzyme™ II PCR Master Mix
Phire® Hot Start II DNA Polymerase DyNAzyme™ I DNA Polymerase
DyNAzyme™ I DNA Polymerase with Mg2+-free buffer
DyNAzyme™ I DNA Polymerase Kit

 

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