Thermo Scientific Finnzymes DNA polymerases

Thermo Scientific product offering for PCR includes a variety of DNA polymerases providing a suitable enzyme for all purposes, from routine PCR to the most demanding high-fidelity applications. The tables below indicate the suitability of different enzymes to specific applications, and some characteristics of the polymerases.

DNA polymerase selection guide

APPLICATIONS
	Phusion® Flash High-Fidelity DNA Polymerase	Phusion® Hot Start II High-Fidelity DNA Polymerase	Phusion® High-Fidelity DNA Polymerase	Phire® Hot Start II DNA Polymerase	DyNAzyme™ EXT DNA Polymerase	DyNAzyme™ II Hot Start DNA Polymerase	DyNAzyme™ II DNA Polymerase	DyNAzyme™ I DNA Polymerase
Direct PCR	+++	+++	+++	+++	–	–	–	–
Fast PCR	+++	+++	+++	+++	+	+	+	+
High-fidelity applications	+++	+++	+++	+	++	+	+	+
Cloning	+++¹	+++¹	+++¹	+¹	++	+	+	+
Difficult templates	+++	+++	+++	++	+++	+	+	+
Long-range PCR	+++	+++	+++	+++	+++	+	++	++
Routine PCR	++	++	++	+++	+++	+++	+++	++
Microarray	+	+++²	+++²	+++²	+++²	+++	++²	++²
Site-directed mutagenesis³	+++	+++	+++	+	++	+	+	+
DHPLC²	+	+++²	+++²	+++²	+++²	+++	++²	++²

CHARACTERISTICS
	Phusion® Flash High-Fidelity DNA Polymerase	Phusion® Hot Start II High-Fidelity DNA Polymerase	Phusion® High-Fidelity DNA Polymerase	Phire® Hot Start II DNA Polymerase	DyNAzyme™ EXT DNA Polymerase	DyNAzyme™ II Hot Start DNA Polymerase	DyNAzyme™ II DNA Polymerase	DyNAzyme™ I DNA Polymerase
5'-3' polymerase activity	Yes	Yes	Yes	Yes	Yes	Yes	Yes	Yes
5'-3' exonuclease activity	No	No	No	No	Yes	Yes	Yes	Yes
3'-5' exonuclease activity	Yes	Yes	Yes	Weak	Weak	No	No	No
Reverse transcriptase activity	No	No	No	No	Low	Low	Low	Low
dUTP incorporation	No	No	No	No	No	Yes	Yes	Yes
Recommended extension time	15 s/kb	15-30 s/kb	15-30 s/kb	10-15 s/kb	40 s/kb	60 s/kb	40 s/kb	40 s/kb

Phusion and Phire DNA polymerases generate blunt end PCR products.
Click here for cloning instructions for Thermo Scientific DNA Polymerases
Detergent-free reaction buffers are available separately for microarray and DHPLC applications, where PCR products are recommended to be free of detergents.
Click here for more information on Polymerase Buffers
For site directed mutagenesis, Finnzymes offers a complete kit:
Phusion® Site-Directed Mutagenesis Kit

Direct links to DNA polymerase products

Phusion® Flash High-Fidelity PCR Master Mix	DyNAzyme™ EXT DNA Polymerase DyNAzyme™ EXT PCR Kit
Phusion® Hot Start II High-Fidelity DNA Polymerase	DyNAzyme™ II Hot Start DNA Polymerase
Phusion® High-Fidelity DNA Polymerase Phusion® High-Fidelity PCR Kit Phusion® High-Fidelity PCR Master Mix	DyNAzyme™ II DNA Polymerase DyNAzyme™ II DNA Polymerase with ^Mg2+-free buffer DyNAzyme™ II DNA Polymerase Kit DyNAzyme™ II PCR Master Mix
Phire® Hot Start II DNA Polymerase	DyNAzyme™ I DNA Polymerase DyNAzyme™ I DNA Polymerase with ^Mg2+-free buffer DyNAzyme™ I DNA Polymerase Kit