DNA - Hind III and ΦX174 DNA - Hae III Mix,
Ready to Use Marker

DNA size standard for broad range

Description

Mix-solution contains both standards: DNA - Hind III digest and ΦX174 DNA - Hae III digest. The solution contains glycerol and bromophenol blue dye and is ready to be pipetted into the gel and used as a DNA size standard.

Fragment Base pairs DNA ΦX174 DNA 1 23,130 2 9,416 3 6,557 4 4,361 5 2,322 6 2,027 7 1,353 8 1,078 9 872 10 603 11 *564 12   310 13a   281 13b   271 14   234 15   194 16a *125 16b   118 17   72

Note: The cohesive ends of fragments 1 and 4 can be separated by heating to 65 °C for 5 minutes.

*Due to the low amount of DNA these bands are almost invisible.

The DNA standard solution contains 19 fragments of the sizes as shown above.

Contents

50 ng/µl DNA supplied in 8 mM Tris-HCl (pH 8.0), 12 mM EDTA, 12 % glycerol, 0.012 % (w/v) bromophenol blue dye.

Storage and stability

For daily use the marker can be stored at +4 °C (1 month). The marker is stable at -20 °C for one year from the assay date.

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