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Phusion® Human Specimen Direct PCR Kit
DNA amplification directly from various human sample types
Description
Finnzymes’ Phusion® Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples. A tiny amount of sample is used directly in the PCR reaction with no prior purification steps. This allows significant savings in both time and cost.
Suitable sample materials include buccal swabs, saliva, amniotic fluid, hair, fingernails, teeth, and skin biopsies. The Phusion Human Specimen Direct PCR Kit kit is compatible with fresh, frozen, and even formalin-fixed, paraffin embedded human tissue samples.
The kit is based on modified Phusion® Hot Start II High-Fidelity DNA Polymerase. This specially engineered proofreading DNA polymerase is fast, robust and highly tolerant of many PCR inhibitors present in human tissues.
The Phusion Human Specimen Direct PCR Kit contains a complete set of optimized reagents and detailed instructions for various sample types. When combined with Finnzymes’ Piko® Thermal Cyclers and UTW® reaction vessels, PCR protocols can be completed in as little as 30 minutes. The kit is for research use only.
Advantages
- No need for time-consuming and expensive DNA purification steps
- Tested with a wide variety of sample types
- Two simple protocols for various applications
- Extremely short PCR protocol times
- Modified Phusion Hot Start II High-Fidelity DNA Polymerase guarantees high yields of specific product
Short protocols, superior performance
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| Consistent results from various human samples with Direct PCR. | Significant time savings and superior performance in comparison to conventional PCR from purified DNA |
Choose direct or dilution protocol based on your needs
Two short and simple protocols for various applications. In the direct protocol, a tiny amount of solid or liquid sample is placed directly into the PCR reaction. In the dilution protocol, the sample is briefly incubated in incubation buffer and 0.5 μl of the supernatant is used as a template in the PCR. The dilution protocol enables numerous PCR reactions to be performed from one tiny sample. For the quickest method from sample to results, choose the direct protocol.
Kit components
Kit component | F-150 |
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Phusion® Human Specimen DNA Polymerase |
80 µl |
2 x Phusion® Human Specimen PCR Buffer (includes dNTPs and MgCl2) |
2 x 1 ml |
Universal control primer mix (25 µM each) |
40 µl |
Dilution Buffer |
2 x 5 ml |
DNARelease™ Additive |
3 x 100 µl |
Storage and stability
Recommended storage temperature -20°C. Stable for one year from the packaging date
Ordering information
Phusion® Human Specimen Direct PCR Kit |
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F-150 |
200 reactions (20 μl each) |
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| Reagents | Instruments | Consumables |
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| No need for DNA purification | ||||||
