Phusion^® Blood Direct PCR Kit

Phusion® Blood Direct PCR Kit

Description

Advantages

Kit components

Storage and stability

Ordering information

For amplification of DNA directly from whole blood.

Note: Starting May 2010 the Phusion Blood DNA polymerase in the kit will be replaced with the new Phusion Blood II DNA Polymerase. The new polymerase allows more flexibility to primer design. No changes are needed to excisting protocols or primers.

Finnzymes’ Phusion^® Blood Direct PCR Kit is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The kit utilizes a modified Phusion^® Hot Start II High-Fidelity DNA Polymerase. This highly processive and robust PCR enzyme is resistant to the inhibitors present in blood and retains its activity even at 40 % blood concentration. Phusion Blood Direct PCR Kit allows analysis of blood samples with different anticoagulants, storage conditions and from various species.

No need for DNA extraction
Allows up to 40 % blood in the PCR reaction
Tolerates heparin, citrate, and EDTA, and is compatible with frozen blood or blood stored on various types of filter papers
Modified Phusion Hot Start II DNA Polymerase gives specific and accurate results with high yields and short protocol times

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Superior performance even at very high blood concentrations	Robust amplification from a wide variety of template sources

Kit component	F-547S	F-547L
Phusion^® Blood II DNA Polymerase	40 µl	200 µl
2 x Phusion^® Blood PCR Buffer (includes dNTPs and MgCl₂)	1 ml	5 x 1 ml
50 mM EDTA	0.5 ml	0.5 ml
50 mM MgCl₂	1.5 ml	1.5 ml
100 % DMSO	0.5 ml	0.5 ml
Universal Control Primer Mix	40 µl	40 µl

Recommended storage temperature -20°C. Stable for one year from the packaging date

Phusion^® Blood Direct PCR Kit
F-547S	100 rxns in 20 µl
F-547L	500 rxns in 20 µl

Tm calculator for PCR Finnzymes' DNA polymerases and cloning


			Instruction manual for Phusion® Blood Direct PCR Kit (with the upgraded DNA polymerase)

			Instruction manual for Phusion® Blood Direct PCR Kit (with the old DNA polymerase)

			Direct PCR brochure

	Application note: Direct PCR from blood preserved on Whatman FTA® and 903® Cards Application note: PCR-RFLP directly from blood

Finnzymes Oy - Keilaranta 16 A, 02150 Espoo, Finland - Tel. +358 9 2472 3010 - Fax +358 9 2472 3200 - fz@finnzymes.fi © Copyright 2010 Thermo Fisher Scientific Inc.

Instruments

Consumables