Phire^® Plant Direct PCR Kit

For amplification of DNA directly from plant material.

Note: Starting January 2010 the Phire Hot Start DNA polymerase in the kit will be replaced with the new Phire Hot Start II DNA Polymerase. The new polymerase allows more flexibility to primer design. No changes are needed to excisting protocols or primers.

Description

Finnzymes’ Phire® Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA isolation is required prior to the PCR. The kit is based on Phire® Hot Start II DNA Polymerase, a specially engineered enzyme with a unique DNA binding domain. Phire DNA Polymerase is extremely robust and tolerant of many PCR inhibitors present in plant material. The kit includes a complete set of optimized reagents, sampling tools and control primers making it an easy and convenient choice for amplification of plant DNA. When combined with Finnzymes’ Piko® Thermal Cycler and UTW® vessels, PCR protocols can be completed in as little as 30 minutes.

Advantages

• No need for DNA extraction
• Phire Hot Start DNA Polymerase features high inhibitor tolerance and allows specificity with abundant yields
• Fast and simple protocols require minimal hands-on time
• Convenient sampling tools included in the kit
• Only very little sample material required
• Tested with leaves and seeds from a wide variety of plant species (see list here; updated March 2010)

Two short protocols for various needs

In the direct protocol, a small piece of plant material is placed directly in the PCR reaction mix. In the dilution protocol, a small volume of the Dilution Buffer containing crushed plant material is added in the PCR reaction mix. The dilution protocol is used for long or difficult amplicons , and for assays where several DNA fragments are amplified from the same sample.

Efficient PCR from various plant leaves and seeds

Click pictures for a full-sized image
Direct PCR from plant leaves	Direct PCR from plant seeds

Which plant materials work with Phire Plant Direct PCR Kit?

Kit components

Kit component F-130 Phire® Hot Start II DNA Polymerase 200 µl 2x Phire® Plant PCR Buffer (includes dNTPs and MgCl2) 5 x 1 ml Control primer mix 40 µl Dilution Buffer 5 ml Harris Uni-Core™ 0.50 mm 1 Harris Cutting Mat™ 1

Storage and stability

Recommended storage temperature -20°C. Stable for six months from the packaging date

Ordering information